Centrifuge-based single cell encapsulation in hydrogel micro...

来自 : 发布时间：2024-05-02

Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples Kiichi Inamori, Hiroaki Onoe, Masahiro Takinoue, Shoji Takeuchi Research output: Chapter in Book/Report/Conference proceeding › Conference contribution Abstract This paper describes a simple, rapid and inexpensive cell encapsulation method using ultra low volume (submicroliter) of cell-suspended pre-gel solution. This method requires only a glass capillary, acrylic holder and tabletop centrifuge, and 20 seconds to complete single cell encapsulation that ensures high viability of encapsulated cells and high efficiency of cell encapsulation. Our cell encapsulation method would be a promising tool for encapsulating rare and precious samples in biological analyses, and would conveniently be acceptable to non-specialists of microfluidics working in biological research and point-of-care medicine. Original languageEnglishTitle of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013PublisherChemical and Biological Microsystems SocietyPages314-316Number of pages3ISBN (Print)9781632666246Publication statusPublished - 2013 Jan 1Externally publishedYesEvent17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, GermanyDuration: 2013 Oct 27 → 2013 Oct 31Publication seriesName17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013Volume1OtherOther17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013Country/TerritoryGermanyCityFreiburgPeriod13/10/27 → 13/10/31 KeywordsCalcium alginateCentrifugeEncapsulationHydrogelSingle cellASJC Scopus subject areasBioengineering Dive into the research topics of Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples . Together they form a unique fingerprint. Inamori, K., Onoe, H., Takinoue, M., Takeuchi, S. (2013). Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 314-316). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society. Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. / Inamori, Kiichi; Onoe, Hiroaki; Takinoue, Masahiro; Takeuchi, Shoji. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 314-316 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution Inamori, K, Onoe, H, Takinoue, M Takeuchi, S 2013, Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 1, Chemical and Biological Microsystems Society, pp. 314-316, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27. Inamori K, Onoe H, Takinoue M, Takeuchi S. Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 314-316. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013). Inamori, Kiichi; Onoe, Hiroaki ; Takinoue, Masahiro ; Takeuchi, Shoji. / Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 314-316 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013). @inproceedings{7d2cf0ec55c344208f0550f9e9f0f776, title = \"Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samples\", abstract = \"This paper describes a simple, rapid and inexpensive cell encapsulation method using ultra low volume (submicroliter) of cell-suspended pre-gel solution. This method requires only a glass capillary, acrylic holder and tabletop centrifuge, and 20 seconds to complete single cell encapsulation that ensures high viability of encapsulated cells and high efficiency of cell encapsulation. Our cell encapsulation method would be a promising tool for encapsulating rare and precious samples in biological analyses, and would conveniently be acceptable to non-specialists of microfluidics working in biological research and point-of-care medicine.\", keywords = \"Calcium alginate, Centrifuge, Encapsulation, Hydrogel, Single cell\", author = \"Kiichi Inamori and Hiroaki Onoe and Masahiro Takinoue and Shoji Takeuchi\", year = \"2013\", month = jan, day = \"1\", language = \"English\", isbn = \"9781632666246\", series = \"17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013\", publisher = \"Chemical and Biological Microsystems Society\", pages = \"314--316\", booktitle = \"17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013\", note = \"17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013\",} TY - GENT1 - Centrifuge-based single cell encapsulation in hydrogel microbeads from ultra low volume of samplesAU - Inamori, KiichiAU - Onoe, HiroakiAU - Takinoue, MasahiroAU - Takeuchi, ShojiPY - 2013/1/1Y1 - 2013/1/1N2 - This paper describes a simple, rapid and inexpensive cell encapsulation method using ultra low volume (submicroliter) of cell-suspended pre-gel solution. This method requires only a glass capillary, acrylic holder and tabletop centrifuge, and 20 seconds to complete single cell encapsulation that ensures high viability of encapsulated cells and high efficiency of cell encapsulation. Our cell encapsulation method would be a promising tool for encapsulating rare and precious samples in biological analyses, and would conveniently be acceptable to non-specialists of microfluidics working in biological research and point-of-care medicine.AB - This paper describes a simple, rapid and inexpensive cell encapsulation method using ultra low volume (submicroliter) of cell-suspended pre-gel solution. This method requires only a glass capillary, acrylic holder and tabletop centrifuge, and 20 seconds to complete single cell encapsulation that ensures high viability of encapsulated cells and high efficiency of cell encapsulation. Our cell encapsulation method would be a promising tool for encapsulating rare and precious samples in biological analyses, and would conveniently be acceptable to non-specialists of microfluidics working in biological research and point-of-care medicine.KW - Calcium alginateKW - CentrifugeKW - EncapsulationKW - HydrogelKW - Single cellUR - http://www.scopus.com/inward/record.url?scp=84907310834 partnerID=8YFLogxKUR - http://www.scopus.com/inward/citedby.url?scp=84907310834 partnerID=8YFLogxKM3 - Conference contributionAN - SCOPUS:84907310834SN - 9781632666246T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013SP - 314EP - 316BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013PB - Chemical and Biological Microsystems SocietyT2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013Y2 - 27 October 2013 through 31 October 2013ER -